Carboxylesterase 1/CES1 Active Protein | CES1 active protein

Recombinant Carboxylesterase 1/CES1

Cat. Num. AAA201130

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Carboxylesterase 1/CES1; Recombinant Carboxylesterase 1/CES1; Liver carboxylesterase 1; CES1G; Ces-1; Ces1; Ses-1; Acyl-coenzyme A:cholesterol acyltransferase; Carboxylesterase 1G; ES-x; CES1 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-565aa
• Sequence: ADP HPSLPPV VHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAAL RLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVV VVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPL AKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTV DFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYP ILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVS DDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYL QIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL HHHHHH
• Sequence Length: 565

• Applicable Applications for CES1 active protein: SDS-PAGE, Enzyme Activity
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Biological Activity: Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for CES1 active protein

Carboxylesterase 1/CES1, also known as liver carboxylesterase 1, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. CES1G shares the serine hydrolase fold observed in other esterases. It is a rat and mouse specific protein that is expressed predominantly in liver, but also in kidney and lung. Recombinant mouse Carboxylesterase 1/CES1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CES1 active protein

Cardiovascular

References

Satoh T., et al. (2006) Chem Biol Interact. 162:195-211. Fleming CD., et al. (2007) Biochemistry. 46:5063-5071.

NCBI and Uniprot Product Information

• NCBI GI #: 162287349
• NCBI Accession #: NP_067431.2
• NCBI GenBank Nucleotide #: NP_067431.2
• UniProt Accession #: Q8VCC2
• Molecular Weight: 61.9kDa (556aa); 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: liver carboxylesterase 1
• UniProt Protein Name: Liver carboxylesterase 1
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: Ces1
• UniProt Synonym Gene Names: Ces1g

Uniprot Description

CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 8 C5|8 45.29 cM

Cellular Component: extracellular space

Biological Process: regulation of gene expression