Ces1d active protein

Ces1d, 19-565aa, Mouse, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206474

• Gene Names: Ces1d; TGH; Ces3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Ces1d; 19-565aa; Mouse; His-tag; Baculovirus (Bioactivity Validated); Carboxylesterase 1D; Ces3; TGH; Ces1d active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL RWVQDNIANF GGNPGSVTIF GESAGGFSVS VLVLSPLAKN LFHRAISESG VSLTAALITT DVKPIAGLVA TLSGCKTTTS AVMVHCLRQK TEDELLETSL KLNLFKLDLL GNPKESYPFL PTVIDGVVLP KAPEEILAEK SFSTVPYIVG INKQEFGWII PTLMGYPLAE GKLDQKTANS LLWKSYPTLK ISENMIPVVA EKYLGGTDDL TKKKDLFQDL MADVVFGVPS VIVSRSHRDA GASTYMYEFE YRPSFVSAMR PKAVIGDHGD EIFSVFGSPF LKDGASEEET NLSKMVMKFW ANFARNGNPN GGGLPHWPEY DQKEGYLKIG ASTQAAQRLK DKEVSFWAEL RAKESAQRPS HREHVELLEH HHHHH
• Sequence Length: 565

• Applicable Applications for Ces1d active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ces1d active protein

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ces1d active protein

Signal Transduction; Metabolism; Enzymes & Proteases

References

Soni KG., et al. (2004) J. Biol. Chem. 279:40683-40689.; Sanghani SP., et al. (2004) Drug Metab Dispos. 32:505-511.

NCBI and Uniprot Product Information

• NCBI GI #: 117553604
• NCBI GeneID: 104158
• NCBI Accession #: NP_444430
• NCBI GenBank Nucleotide #: NM_053200.2
• UniProt Accession #: Q8VCT4; Q91ZV9; Q924V8; Q9ULY1
• Molecular Weight: 60.9kDa (555aa) 50-70KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: carboxylesterase 1D
• NCBI Official Synonym Full Names: carboxylesterase 1D
• NCBI Official Symbol: Ces1d
• NCBI Official Synonym Symbols: TGH; Ces3
• NCBI Protein Information: carboxylesterase 1D
• UniProt Protein Name: Carboxylesterase 1D
• Protein Family: Carboxylesterase
• UniProt Gene Name: Ces1d
• UniProt Synonym Gene Names: FAEE synthase; TGH

Uniprot Description

Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters.