Mouse anti-Human CES1 Monoclonal Antibody | anti-Ces1e antibody

CES1 (Carboxylesterase 1, Acyl Coenzyme A:Cholesterol Acyltransferase, ACAT, Brain Carboxylesterase hBr1, CEH, Egasyn, HMSE, HMSE1, Liver Carboxylesterase 1, MGC117365, Monocyte/macrophage Serine Esterase 1, PCE-1, Serine Esterase 1, SES1, Triacylglycerol

Cat. Num. AAA646986

• Gene Names: Ces1e; Ces1; Es22
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: CES1; Monoclonal Antibody; CES1 (Carboxylesterase 1; Acyl Coenzyme A:Cholesterol Acyltransferase; ACAT; Brain Carboxylesterase hBr1; CEH; Egasyn; HMSE; HMSE1; Liver Carboxylesterase 1; MGC117365; Monocyte/macrophage Serine Esterase 1; PCE-1; Serine Esterase 1; SES1; Triacylglycerol; Anti -CES1 (Carboxylesterase 1; anti-Ces1e antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 11n45
• Specificity: Recognizes human CES-1. Species Crossreactivity: recombinant mouse CES-3. No cross-reactivity with rmCES-2, rmCES-5, or recombinant human CES-2.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-Ces1e antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 2ug/ml; Immunoprecipitation: 25ug/ml
• Immunogen: Recombinant corresponding to aa19-563 from human CES-1 expressed in NS0 cells (P23141).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human Carboxylesterase 1/CES1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 μg/mL of Human Carboxylesterase 1/CES1 Monoclonal Antibody followed by HRP­conjugated Mouse IgG Secondary Antibody. A specific band was detected for Carboxylesterase 1/CES1 at approximately 70kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-Ces1e antibody

Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES-1 shares the serine hydrolase fold observed in other esterases (3). CES-1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus.

Product Categories/Family for anti-Ces1e antibody

Antibodies; Abs to Enzymes; Acyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 298493214
• NCBI GeneID: 29225
• NCBI Accession #: NP_113753.2
• NCBI GenBank Nucleotide #: NM_031565.2
• UniProt Accession #: Q63108
• Molecular Weight: 61,715 Da
• NCBI Official Full Name: carboxylesterase 1E
• NCBI Official Synonym Full Names: carboxylesterase 1E
• NCBI Official Symbol: Ces1e
• NCBI Official Synonym Symbols: Ces1; Es22
• NCBI Protein Information: carboxylesterase 1E; egasyn; ES-HTEL; esterase 22; pI 5.5 esterase; carboxyesterase ES-3; liver carboxylesterase 3
• UniProt Protein Name: Carboxylesterase 1E
• Protein Family: Carboxylesterase
• UniProt Gene Name: Ces1e
• UniProt Synonym Gene Names: Ces1
• UniProt Entry Name: EST1E_RAT

NCBI Description

enzyme that hydrolyzes long-chain fatty acids; necessary for cholesterol esterification; may be important for detoxification of certain drugs [RGD, Feb 2006]

Uniprot Description

Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.

Catalytic activity: A carboxylic ester + H2O = an alcohol + a carboxylate.

Subcellular location: Endoplasmic reticulum lumen. Note: Microsomal membrane, lumen of endoplasmic reticulum.

Sequence similarities: Belongs to the type-B carboxylesterase/lipase family.