Western Blot (WB)
(Western blot using Immunochemicals anti- hCE1 shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66kD band. Purified hCE1 (lane 4) is similarly detected. The higher molecular weight band in lane 4 may represent a trimeric form of hCE1. No reactivity is seen in the untransfected control (lane 1). No cross reactivity occurs against the human intestinal form of CES1 (lane 3. The primary antibody was used at a 1:2,500 dilution to detect purified hCE1.)
Rabbit CES1 Polyclonal Antibody | anti-CES1 antibody
CES1 (Carboxylesterase 1, ACAT, CEH, Monocyte/macrophage serine esterase 1, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn)
Gene Names
CES1; CES; MGC142628
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CES1; Polyclonal Antibody; CES1 (Carboxylesterase 1; ACAT; CEH; Monocyte/macrophage serine esterase 1; HMSE; HMSE1; MGC117365; PCE-1; SES1; TGH; acyl coenzyme A:cholesterol acyltransferase; carboxylesterase 1; carboxylesterase 2 (liver); cholesteryl ester hydrolase; egasyn); Anti -CES1 (Carboxylesterase 1; anti-CES1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Applicable Applications for anti-CES1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: Western Blot: 1:2,500-1:5,000
Immunogen
Recombinant human Liver Carboxylesterase-1
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot using Immunochemicals anti- hCE1 shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66kD band. Purified hCE1 (lane 4) is similarly detected. The higher molecular weight band in lane 4 may represent a trimeric form of hCE1. No reactivity is seen in the untransfected control (lane 1). No cross reactivity occurs against the human intestinal form of CES1 (lane 3. The primary antibody was used at a 1:2,500 dilution to detect purified hCE1.)
Western Blot (WB)
(Western blot using Immunochemicals anti- hCE1 shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66kD band. Purified hCE1 (lane 4) is similarly detected. The higher molecular weight band in lane 4 may represent a trimeric form of hCE1. No reactivity is seen in the untransfected control (lane 1). No cross reactivity occurs against the human intestinal form of CES1 (lane 3. The primary antibody was used at a 1:2,500 dilution to detect purified hCE1.)
Related Product Information for anti-CES1 antibody
Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Product Categories/Family for anti-CES1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~66kD
NCBI Official Full Name
carboxylesterase 1
NCBI Official Symbol
CES1
NCBI Official Synonym Symbols
CES; MGC142628
NCBI Protein Information
carboxylesterase 1
UniProt Protein Name
Carboxylesterase 1 (Monocyte/macrophage serine esterase 1)
Protein Family
UniProt Gene Name
CES1
UniProt Entry Name
Q0VCI3_BOVIN
Uniprot Description
Sequence similarities: Belongs to the type-B carboxylesterase/lipase family. RuleBase RU000349V1
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Product Notes
The CES1 ces1 (Catalog #AAA622732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CES1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: Western Blot: 1:2,500-1:5,000. Researchers should empirically determine the suitability of the CES1 ces1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CES1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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