Carboxylesterase 1/CES1 Active Protein | CES1 active protein

Recombinant Carboxylesterase 1/CES1

Cat. Num. AAA201127

• Gene Names: CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Carboxylesterase 1/CES1; Recombinant Carboxylesterase 1/CES1; Liver carboxylesterase 1 isoform a; CES1; ACAT; CE-1; CEH; CES2; hCE-1; HMSE; HMSE1; PCE-1; REH; SES1; TGH; CES1 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 25mM Sodium Acetate (pH 4.0) Containing 10% Glycerol, 0.1M NaCl, 0.1mM PMSF.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-568aa
• Sequence: ADLGHPSSPP VVDTVHGKVL GKFVSLEGFA QPVAIFLGIP FAKPPLGPLR FTPPQPAEPW SFVKNATSYP PMCTQDPKAG QLLSELFTNR KENIPLKLSE DCLYLNIYTP ADLTKKNRLP VMVWIHGGGL MVGAASTYDG LALAAHENVV VVTIQYRLGI WGFFSTGDEH SRGNWGHLDQ VAALRWVQDN IASFGGNPGS VTIFGESAGG ESVSVLVLSP LAKNLFHRAI SESGVALTSV LVKKGDVKPL AEQIAITAGC KTTTSAVMVH CLRQKTEEEL LETTLKMKFL SLDLQGDPRE SQPLLGTVID GMLLLKTPEE LQAERNFHTV PYMVGINKQE FGWLIPMQLM SYPLSEGQLD QKTAMSLLWK SYPLVCIAKE LIPEATEKYL GGTDDTVKKK DLFLDLIADV MFGVPSVIVA RNHRDAGAPT YMYEFQYRPS FSSDMKPKTV IGDHGDELFS VFGAPFLKEG ASEEEIRLSK MVMKFWANFA RNGNPNGEGL PHWPEYNQKE GYLQIGANTQ AAQKLKDKEV AFWTNLFAKK AVEKPPQTEH IELHHHHHH
• Sequence Length: 567

• Applicable Applications for CES1 active protein: Enzyme Activity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Biological Activity: Specific activity is > 700 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for CES1 active protein

Carboxylesterase 1, also known as liver carboxylesterase 1 isoform a, is a member of a large family of carboxylesterases. It is also part of the alpha/beta fold hydrolase family. Carboxylesterases hydrolyze long-chain fatty acid esters and thioesters. The resulting carboxylates are then often conjugated by other enzymes to increase solubility and eventually excreted. Also, this enzyme may play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. This protein is known to react aromatic and aliphatic esters and can manage cellular cholesterol esterification levels. It is present in most tissues with higher levels in the liver and low levels in the gastrointestinal tract. Recombinant human Carboxylesterase 1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CES1 active protein

Cardiovascular

References

Satoh T., et al, (2006) Chem Biol Interact. 162:195-211. Redinbo MR., et al. (2005) Drug Discovery Today 10:313.

NCBI and Uniprot Product Information

• NCBI GI #: 119576
• NCBI GeneID: 1066
• UniProt Accession #: P23141
• Molecular Weight: 61.7kDa (559aa); 50-70kDa (SDS-PAGE under non-reducing conditions)
• NCBI Official Full Name: Liver carboxylesterase 1
• NCBI Official Synonym Full Names: carboxylesterase 1
• NCBI Official Symbol: CES1
• NCBI Official Synonym Symbols: CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
• NCBI Protein Information: liver carboxylesterase 1
• UniProt Protein Name: Liver carboxylesterase 1
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: CES1
• UniProt Synonym Gene Names: CES2; SES1; ACAT; CE-1; hCE-1; REH; TGH
• UniProt Entry Name: EST1_HUMAN

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.56; EC 3.1.1.1

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: epithelial cell differentiation; metabolic process; response to toxin