SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Arsa active protein
Recombinant Arsa
Gene Names
Arsa; ASA; As2; AS-A; As-2; TISP73; AW212749
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
Arsa; Recombinant Arsa; Arylsulfatase A; As-2; AS-A; As2; ASA; AW212749; TISP73; Arsa active protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
18-506aa
Sequence
ADPSPPNILL IFADDLGYGD LGSYGHPSST TPNLDQLAEG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRS GMYPGVLGPS SQGGLPLEEV TLAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPD IPCKGGCDQG LVPIPLLANL TVEAQPPWLP GLEARYVSFS RDLMADAQRQ GRPFFLYYAS HHTHYPQFSG QSFTKRSGRG PFGDSLMELD GAVGALMTTV GDLGLLEETL VIFTADNGPE LMRMSNGGCS GLLRCGKGTT FEGGVREPAL VYWPGHITPG VTHELASSLD LLPTLAALTG APLPNVTLDG VDISPLLLGT GKSPRKSVFF YPPYPDEIHG VFAVRNGKYK AHFFTQGSAH SDTTSDPACH AANRLTAHEP PLLYDLSQDP GENYNVLESI EGVSPEALQA LKHIQLLKAQ YDAAMTFGPS QIAKGEDPAL QICCQPSCTP HPVCCHCPGS QSHHHHHH
Sequence Length
506
Applicable Applications for Arsa active protein
Enzyme Activity, SDS-PAGE
Species
Mouse
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ala
Biological Activity
Specific activity is > 4,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for Arsa active protein
Arsa, also known as arylsulfatase A, is a prototype member of the sulfatase family. It is an enzyme that catalyzes the degradation of sulfatides, a glycosphingolipid found in many tissues, but predominantly in myelin and kidney. The serum level of its might be helpful in identification of lung and central nervous system cancer. Recombinant mouse Arsa, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Arsa active protein
References
Lukatela G., et al. (1998) Biochemistry. 37:3654-3664. Jean S., et al. (2006) Alcohol Clin Exp Res. 30:1950-1955.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53.2kDa (498aa); 50-70kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
arylsulfatase A
NCBI Official Synonym Full Names
arylsulfatase A
NCBI Official Symbol
Arsa
NCBI Official Synonym Symbols
ASA; As2; AS-A; As-2; TISP73; AW212749
NCBI Protein Information
arylsulfatase A
UniProt Protein Name
Arylsulfatase A
Protein Family
UniProt Gene Name
Arsa
UniProt Synonym Gene Names
As2; ASA
Uniprot Description
Hydrolyzes cerebroside sulfate.
Research Articles on Arsa
Similar Products
Product Notes
The Arsa arsa (Catalog #AAA201096) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 18-506aa. AAA Biotech's Arsa can be used in a range of immunoassay formats including, but not limited to, Enzyme Activity, SDS-PAGE. Researchers should empirically determine the suitability of the Arsa arsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPSPPNILL IFADDLGYGD LGSYGHPSST TPNLDQLAEG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRS GMYPGVLGPS SQGGLPLEEV TLAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPD IPCKGGCDQG LVPIPLLANL TVEAQPPWLP GLEARYVSFS RDLMADAQRQ GRPFFLYYAS HHTHYPQFSG QSFTKRSGRG PFGDSLMELD GAVGALMTTV GDLGLLEETL VIFTADNGPE LMRMSNGGCS GLLRCGKGTT FEGGVREPAL VYWPGHITPG VTHELASSLD LLPTLAALTG APLPNVTLDG VDISPLLLGT GKSPRKSVFF YPPYPDEIHG VFAVRNGKYK AHFFTQGSAH SDTTSDPACH AANRLTAHEP PLLYDLSQDP GENYNVLESI EGVSPEALQA LKHIQLLKAQ YDAAMTFGPS QIAKGEDPAL QICCQPSCTP HPVCCHCPGS QSHHHHHH. It is sometimes possible for the material contained within the vial of "Arsa, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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