Rabbit anti-Mouse ARSA Polyclonal Antibody | anti-ARSA antibody

Anti-ARSA Antibody, Rabbit Polyclonal

Cat. Num. AAA8110781

• Gene Names: Arsa; ASA; As2; AS-A; As-2; TISP73; AW212749
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ARSA; Polyclonal Antibody; Anti-ARSA Antibody; Rabbit Polyclonal; Arylsulfatase A/ARSA Antibody; Rabbit PAb; Antigen Affinity Purified; arylsulfatase A; Anti-As-2 Antibody; Anti-AS-A Antibody; Anti-As2 Antibody; Anti-ASA Antibody; Anti-AW212749 Antibody; Anti-TISP73 Antibody; anti-ARSA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Arylsulfatase-A/ARSA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ARSA antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 4-6uL/mg of lysate
• Immunogen: Recombinant Mouse ARSA protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse ARSA (rM ARSA). ARSA specific IgG was purified by Mouse ARSA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(Mouse ARSA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-Mouse ARSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse ARSA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size: 54 kDa)

Western Blot (WB)

(Anti-ARSA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-ARSA antibody

Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co-or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in identification of lung and central nervous system cancer.

References

Laidler PM. (1991) Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis Folia Med Cracov. 32(3-4): 149-68.Jean S, et al. (2006) Ethanol decreases rat hepatic arylsulfatase A activity levels. Alcohol Clin Exp Res. 30(11): 1950-5.

NCBI and Uniprot Product Information

• NCBI GI #: 67972647
• NCBI GeneID: 11883
• NCBI Accession #: NP_033843.2
• NCBI GenBank Nucleotide #: NM_009713.4
• UniProt Accession #: P50428; Q9DC66
• Molecular Weight: 53,748 Da
• NCBI Official Full Name: arylsulfatase A
• NCBI Official Synonym Full Names: arylsulfatase A
• NCBI Official Symbol: Arsa
• NCBI Official Synonym Symbols: ASA; As2; AS-A; As-2; TISP73; AW212749
• NCBI Protein Information: arylsulfatase A; cerebroside-sulfatase
• UniProt Protein Name: Arylsulfatase A
• Protein Family: Arylsulfatase
• UniProt Gene Name: Arsa
• UniProt Synonym Gene Names: As2; ASA

Uniprot Description

Hydrolyzes cerebroside sulfate.