ARSA active protein

ARSA Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122466

• Gene Names: Arsa; ASA; As2; AS-A; As-2; TISP73; AW212749
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ARSA; ARSA Protein; Mouse; Recombinant (His Tag); Mouse Arylsulfatase A/ARSA Protein (His Tag); As-2 Protein; AS-A Protein; As2 Protein; ASA Protein; AW212749 Protein; TISP73 Protein; arylsulfatase A; ARSA active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser506

• Species: Mouse
• Activity: Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ser 506) of mouse ARSA (NP_033843.2) precursor was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ARSA active protein

Background: Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in test of lung and central nervous system cancer.

References

Laidler PM. (1991) Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis Folia Med Cracov. 32(3-4): 149-68.Jean S, et al. (2006) Ethanol decreases rat hepatic arylsulfatase A activity levels. Alcohol Clin Exp Res. 30(11): 1950-5.

NCBI and Uniprot Product Information

• NCBI GI #: 67972647
• NCBI GeneID: 11883
• NCBI Accession #: NP_033843.2
• NCBI GenBank Nucleotide #: NM_009713.4
• UniProt Accession #: P50428; Q9DC66
• Molecular Weight: 53,748 Da
• NCBI Official Full Name: arylsulfatase A
• NCBI Official Synonym Full Names: arylsulfatase A
• NCBI Official Symbol: Arsa
• NCBI Official Synonym Symbols: ASA; As2; AS-A; As-2; TISP73; AW212749
• NCBI Protein Information: arylsulfatase A; cerebroside-sulfatase
• UniProt Protein Name: Arylsulfatase A
• Protein Family: Arylsulfatase
• UniProt Gene Name: Arsa
• UniProt Synonym Gene Names: As2; ASA

Uniprot Description

Hydrolyzes cerebroside sulfate.