BACE1 recombinant protein

BACE1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118824

• Gene Names: Bace1; ASP2; Bace; C76936
• Purity: >97 % as determined by SDS-PAGE
• Synonyms: BACE1; BACE1 Protein; Mouse; Recombinant (His Tag); Mouse BACE-1 Protein (His Tag); beta-site APP-cleaving enzyme 1; C76936 Protein; BACE1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 447aa
• Sequence: Met1-Thr457

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr22
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Thr 457) of mouse BACE1 (NM_011792.5) precursor was expressed with a C-terminal polyhistidine tag
• Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2. Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.; The specific activity is >2 pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for BACE1 recombinant protein

Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

References

Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.

NCBI and Uniprot Product Information

• NCBI GI #: 225543119
• NCBI GeneID: 23821
• NCBI Accession #: NM_011792.5
• NCBI GenBank Nucleotide #: NM_011792.5
• UniProt Accession #: P56818; Q544D0
• Molecular Weight: 55,748 Da
• NCBI Official Full Name: Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: beta-site APP cleaving enzyme 1
• NCBI Official Symbol: Bace1
• NCBI Official Synonym Symbols: ASP2; Bace; C76936
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: Bace1
• UniProt Synonym Gene Names: Bace; ASP2; Asp 2; Beta-site APP cleaving enzyme 1

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality. [provided by RefSeq, Nov 2015]

Uniprot Description

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase ().