Rabbit anti-Mouse BACE1 Polyclonal Antibody | anti-BACE1 antibody

Anti-BACE1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110742

• Gene Names: Bace1; ASP2; Bace; C76936
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: BACE1; Polyclonal Antibody; Anti-BACE1 Antibody; Rabbit Polyclonal; BACE1 Antibody; Rabbit PAb; Antigen Affinity Purified; beta-site APP-cleaving enzyme 1; Anti-C76936 Antibody; anti-BACE1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse BACE1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BACE1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Mouse BACE1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse BACE1 (rM BACE1). BACE1 specific IgG was purified by Mouse BACE1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse BACE1 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-BACE1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDaObserved band size:70 kDa)

Related Product Information for anti-BACE1 antibody

Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

References

Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.

NCBI and Uniprot Product Information

• NCBI GI #: 225543119
• NCBI GeneID: 23821
• NCBI Accession #: NM_011792.5
• NCBI GenBank Nucleotide #: NM_011792.5
• UniProt Accession #: P56818; Q544D0
• Molecular Weight: 55,748 Da
• NCBI Official Full Name: Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: beta-site APP cleaving enzyme 1
• NCBI Official Symbol: Bace1
• NCBI Official Synonym Symbols: ASP2; Bace; C76936
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: Bace1
• UniProt Synonym Gene Names: Bace; ASP2; Asp 2; Beta-site APP cleaving enzyme 1

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality. [provided by RefSeq, Nov 2015]

Uniprot Description

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase ().