BACE1 active protein

BACE1 Protein, Human, Recombinant

Cat. Num. AAA8119979

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BACE1; BACE1 Protein; Human; Recombinant; Human BACE1/ASP2 Protein; ASP2 Protein; BACE Protein; HSPC104 Protein; beta-site APP-cleaving enzyme 1; BACE1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr457

• Source: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 22
• Tag: C-cleavage
• Protein Construction: The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Bio Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2. Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405nM, respectively. The specific activity is >3.5pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BACE1 active protein

Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+)(microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

References

Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.

NCBI and Uniprot Product Information

• NCBI GI #: 6912266
• NCBI GeneID: 23621
• NCBI Accession #: NP_036236.1
• NCBI GenBank Nucleotide #: NM_012104.4
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 42,107 Da
• NCBI Official Full Name: beta-secretase 1 isoform A preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis