Mouse Tubulin Alpha 4A (TUBa4A) ELISA Kit | TUBa4A elisa kit

Mouse Tubulin Alpha 4A (TUBa4A) ELISA Kit

Cat. Num. AAA2705589

• Gene Names: TUBA4A; ALS22; TUBA1; H2-ALPHA
• Reactivity: Mouse
• Synonyms: Tubulin Alpha 4A (TUBa4A); Mouse Tubulin Alpha 4A (TUBa4A) ELISA Kit; TUBA1; Testis Specific Tubulin Alpha 1; Alpha-tubulin 1; Tubulin alpha-1 chain; Tubulin H2-alpha; TUBa4A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tubulin Alpha 4A (TUBa4A).; No significant cross-reactivity or interference between Tubulin Alpha 4A (TUBa4A) and analogues was observed.
• Sequence Length: 433

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Alpha 4A (TUBa4A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Alpha 4A (TUBa4A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBa4A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Alpha 4A (TUBa4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Alpha 4A (TUBa4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Alpha 4A (TUBa4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tubulin Alpha 4A (TUBa4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14328047
• NCBI GeneID: 7277
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: 48,329 Da
• NCBI Official Full Name: Tubulin, alpha 4a
• NCBI Official Synonym Full Names: tubulin alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: ALS22; TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.