Rabbit GOPC Polyclonal Antibody | anti-GOPC antibody

GOPC Rabbit Polyclonal

Cat. Num. AAA769794

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: GOPC; Polyclonal Antibody; GOPC Rabbit Polyclonal; anti-GOPC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 462

• Applicable Applications for anti-GOPC antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Golgi associated PDZ and coiled-coil motif containing
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS769794 (GOPC antibody) at dilution of 1:50)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS769794 (GOPC antibody) at dilution of 1:800)

NCBI and Uniprot Product Information

• NCBI GI #: 9966877
• NCBI GeneID: 57120
• NCBI Accession #: NP_065132.1
• NCBI GenBank Nucleotide #: NM_020399.3
• Molecular Weight: 35,130 Da
• NCBI Official Full Name: Golgi-associated PDZ and coiled-coil motif-containing protein isoform a
• NCBI Official Synonym Full Names: golgi associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG; PIST
• UniProt Entry Name: GOPC_HUMAN

NCBI Description

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell junction; cytoplasm; dendrite; Golgi apparatus; lysosomal membrane; membrane; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; trans-Golgi network transport vesicle

Molecular Function: protein binding

Biological Process: apical protein localization; cytoplasmic sequestering of CFTR protein; ER to Golgi vesicle-mediated transport; Golgi to plasma membrane transport; protein homooligomerization; protein transport

Disease: Spermatogenic Failure 6