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Western Blot (WB) (Western blot analysis of SKOV3 Hela cell Mouse liver tissue Mouse heart tissue Human placenta tissue HepG2 cell Human heart tissue lysates using ATP5O Polyclonal Antibody at dilution of 1:1250)

Rabbit anti-Human, Mouse ATP5O Polyclonal Antibody | anti-ATP5O antibody

ATP5O Polyclonal Antibody

Gene Names
ATP5PO; ATPO; OSCP; ATP5O; HMC08D05
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purification
Synonyms
ATP5O; Polyclonal Antibody; ATP5O Polyclonal Antibody; ATP synthase O subunit mitochondrial precursor; ATP synthase subunit O; ATP synthase; H+ transporting; mitochondrial F1 complex; O subunit; ATPO; Mitochondrial; Mitochondrial ATP synthase; Oligomycin sensitivity conferral protein; OSCP; anti-ATP5O antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Concentration
1.56mg/mL (varies by lot)
Sequence Length
213
Applicable Applications for anti-ATP5O antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-1:5000
IHC: 1:100-1:300
ELISA: 1:5000-1:10000
Immunogen
Fusion protein of human ATP5PO
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of SKOV3 Hela cell Mouse liver tissue Mouse heart tissue Human placenta tissue HepG2 cell Human heart tissue lysates using ATP5O Polyclonal Antibody at dilution of 1:1250)

Western Blot (WB) (Western blot analysis of SKOV3 Hela cell Mouse liver tissue Mouse heart tissue Human placenta tissue HepG2 cell Human heart tissue lysates using ATP5O Polyclonal Antibody at dilution of 1:1250)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ATP5O Polyclonal Antibody at dilution of 1:90(×200))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ATP5O Polyclonal Antibody at dilution of 1:90(×200))
Related Product Information for anti-ATP5O antibody
The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
Product Categories/Family for anti-ATP5O antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
539
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23 kDa
NCBI Official Full Name
ATP synthase subunit O, mitochondrial
NCBI Official Synonym Full Names
ATP synthase peripheral stalk subunit OSCP
NCBI Official Symbol
ATP5PO
NCBI Official Synonym Symbols
ATPO; OSCP; ATP5O; HMC08D05
NCBI Protein Information
ATP synthase subunit O, mitochondrial
UniProt Protein Name
ATP synthase subunit O, mitochondrial
UniProt Gene Name
ATP5O
UniProt Synonym Gene Names
ATPO; OSCP
UniProt Entry Name
ATPO_HUMAN

NCBI Description

The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5O: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase delta chain family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: mitochondrion; mitochondrial inner membrane; plasma membrane; nucleus; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; drug binding; transmembrane transporter activity

Biological Process: cellular metabolic process; proton transport; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport

Research Articles on ATP5O

Similar Products

Product Notes

The ATP5O atp5o (Catalog #AAA2562992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5O Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5O can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-1:5000 IHC: 1:100-1:300 ELISA: 1:5000-1:10000. Researchers should empirically determine the suitability of the ATP5O atp5o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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