Human Growth Hormone Receptor (GHR) ELISA Kit | GHR elisa kit

Human Growth Hormone Receptor (GHR) ELISA Kit

Cat. Num. AAA451440

• Gene Names: GHR; GHBP; GHIP
• Reactivity: Human
• Synonyms: Growth Hormone Receptor (GHR); Human Growth Hormone Receptor (GHR) ELISA Kit; GHBP Somatotropin receptor Growth hormone-binding protein Serum-binding protein; GHR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GHR. No significant cross-reactivity or interference between GHR and analogues was observed.
• Sequence Length: 638

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.62ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GHR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GHR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GHR elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GHR in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GHR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GHR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GHR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503993
• NCBI GeneID: 2690
• NCBI Accession #: NP_000154.1
• NCBI GenBank Nucleotide #: NM_000163.4
• UniProt Accession #: P10912; Q9HCX2
• Molecular Weight: 69,237 Da
• NCBI Official Full Name: growth hormone receptor isoform 1
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: GHR
• NCBI Official Synonym Symbols: GHBP; GHIP
• NCBI Protein Information: growth hormone receptor
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Growth hormone receptor
• UniProt Gene Name: GHR
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP

NCBI Description

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway ().