Rabbit anti-Human, Mouse Glutaredoxin-2 Polyclonal Antibody | anti-GLRX2 antibody

Glutaredoxin-2, Mitochondrial, CT, T135 (GLRX2, GRX2, CGI-133) (MaxLight 650)

Cat. Num. AAA6478217

• Gene Names: GLRX2; GRX2; CGI-133
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Glutaredoxin-2; Polyclonal Antibody; Mitochondrial; CT; T135 (GLRX2; GRX2; CGI-133) (MaxLight 650); anti-GLRX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GLRX2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-GLRX2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GLRX2 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GLRX2 antibody

Antibodies; Proteins

References

1. Lundberg M., Johansson C. J. Biol. Chem. 276:26269-26275(2001). 2. Gladyshev V.N., Liu A. J. Biol. Chem. 276:30374-30380(2001). 3. Lillig C.H. Proc. Natl. Acad. Sci. USA 101:13227-13232(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 37537704
• NCBI GeneID: 51022
• NCBI Accession #: NP_932066
• NCBI GenBank Nucleotide #: NM_197962.2
• UniProt Accession #: Q9NS18; Q3LR69; Q7L1N7; Q96JC0; Q9Y3D4
• Molecular Weight: 17.0 kDa (154aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: glutaredoxin 2 isoform 2
• NCBI Official Synonym Full Names: glutaredoxin 2
• NCBI Official Symbol: GLRX2
• NCBI Official Synonym Symbols: GRX2; CGI-133
• NCBI Protein Information: glutaredoxin 2
• UniProt Protein Name: Glutaredoxin-2, mitochondrial
• Protein Family: Glutaredoxin
• UniProt Gene Name: GLRX2
• UniProt Synonym Gene Names: GRX2
• UniProt Entry Name: GLRX2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011]