Typical Testing Data/Standard Curve (for reference only)
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Alanine Aminotransferase (ALT/GPT) Assay Kit | ALT/GPT assay kit
Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit
Synonyms
Alanine Aminotransferase (ALT/GPT); Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit; ALT; GPT; Alanine aminotransferase; glutamate pyruvic transaminase; ALT/GPT assay kit
Samples
Serum, plasma, animal and plant tissues, cells, bacteria
Preparation and Storage
Storage at 4 degree C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for ALT/GPT assay kit
Principle of the Assay: Alanine Aminotransferase (ALT/GPT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, ALT has the highest content in cardiomyocytes, and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as Animal Tissues, Plant Tissues, Cell culture (Adherent or Suspension Cells), Serum, Bacteria. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37 degree C and pH7.4 to produce Glutamic Acid and Pyruvate; Pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate Phenylhydrazone, which appears brownish red under Alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of Pyruvate Phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.
Product Categories/Family for ALT/GPT assay kit
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Product Notes
The ALT/GPT (Catalog #AAA9719084) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Alanine Aminotransferase (ALT/GPT), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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