General Total Bile Acid (TBA) Assay Kit | TBA assay kit
Total Bile Acid (TBA) Colorimetric Assay Kit
Reactivity
General
Synonyms
Total Bile Acid (TBA); Total Bile Acid (TBA) Colorimetric Assay Kit; TBA assay kit
Reactivity
General
Samples
Serum samples
Detection Range
2.05 umol/L
Sensitivity
2.05-120 umol/L
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for TBA assay kit
Intended Uses: The kit can be used to detect the concentration of total bile acid (TBA) in serum samples.
Principle of the Assay: With S-NAD+ as hydrogen receptor, 3alpha-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3alpha-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Background/Introduction: Bile acids usually consist of a 24-carbon steroid core and a side chain with a carboxyl group. The catabolites of mammalian cholesterol are primarily primary bile acids, including cholic and deoxycholic acids, which are secreted into bile in combination with taurine or glycine. The primary bile acids are dehydroxylated and converted to secondary bile acids, including deoxycholic and lithocholic acids. Metabolism disorder of bile acid is related to liver disease, inflammatory bowel disease, non-alcoholic fatty liver disease, diabetes, obesity and other diseases.
Principle of the Assay: With S-NAD+ as hydrogen receptor, 3alpha-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3alpha-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Background/Introduction: Bile acids usually consist of a 24-carbon steroid core and a side chain with a carboxyl group. The catabolites of mammalian cholesterol are primarily primary bile acids, including cholic and deoxycholic acids, which are secreted into bile in combination with taurine or glycine. The primary bile acids are dehydroxylated and converted to secondary bile acids, including deoxycholic and lithocholic acids. Metabolism disorder of bile acid is related to liver disease, inflammatory bowel disease, non-alcoholic fatty liver disease, diabetes, obesity and other diseases.
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Product Notes
The General TBA (Catalog #AAA2563699) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2563699 Assay Kit recognizes General TBA. It is sometimes possible for the material contained within the vial of "Total Bile Acid (TBA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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