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Immunocytochemistry/Immunofluorescence (ICC/IF))
Eukaryotes Protein Synthesis Assay Kit
EZClick Global Protein Synthesis Assay Kit
Reactivity
Eukaryotes
Synonyms
Protein Synthesis; EZClick Global Protein Synthesis Assay Kit; Protein Synthesis assay kit
Reactivity
Eukaryotes
Samples
Suspension or adherent cell cultures
Detection Method
Fluorescence: Ex/Em 494/521nm
Features & Benefits
Simple, fast, does not require lengthy incubation times
This assay provides a convenient and accurate procedure to measure de novo protein synthesized in biological samples
Microplate-based assay to detect cell proliferation using metabolic labeling techniques
This assay provides a convenient and accurate procedure to measure de novo protein synthesized in biological samples
Microplate-based assay to detect cell proliferation using metabolic labeling techniques
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Protein Synthesis assay kit
Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc). It is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical in determining the physiological state of the cell. Methods of detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important for assessment of cytotoxicity. EZClick Global Protein Synthesis Assay Kit utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em= 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in the real-time, and Cycloheximide, an inhibitor of protein synthesis that serves as a control
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Product Notes
The Eukaryotes Protein Synthesis (Catalog #AAA849687) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849687 Assay Kit recognizes Eukaryotes Protein Synthesis. It is sometimes possible for the material contained within the vial of "Protein Synthesis, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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