Radius 96-Well Cell Migration Assay Assay Kit

Radius 96-Well Cell Migration Assay

Cat. Num. AAA168787

• Synonyms: Radius 96-Well Cell Migration Assay; Radius 96-Well Cell Migration Assay assay kit

• Preparation and Storage: Upon receipt, aliquot and store the Radius Gel Removal Solution and DAPI Fluorescence Stain at -20 degree C (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4 degree C. All other kit components should be stored at room temperature until the kit's expiration date.

Testing Data

Testing Data #2

Related Product Information for Radius 96-Well Cell Migration Assay assay kit

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). The Radius Cell Migration Assay Kit utilizes a proprietary 96-well plate to monitor the migratory properties of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot (Radius Gel) where cells cannot attach. When adherent cells are seeded in the Radius Cell Migration well, they attach outside of the Radius Gel coated area. Once firm cell attachment is achieved, the hydrogel is quickly removed to expose a cell-free region to study cell migration/closure. This format provides a robust in vitro system to measure 2-D cell migration, screen potential inhibitors and study cytoskeleton reorganization events. The Radius Cell Migration Assay Kit is designed for High Content Analysis applications or imaging software and is adaptable to liquid handling equipment. Each kit provides sufficient quantities to perform 96 migration tests.

Product Categories/Family for Radius 96-Well Cell Migration Assay assay kit

Cell Based Assays; Cell Migration; Invasion and Wound Healing; Gap Closure Assays; Cell Migration Assays; 2D Gap Closure

References

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