Radius 24-Well Cell Migration Assay, (ECM Array Coated) Assay Kit

Radius 24-Well Cell Migration Assay, (ECM Array Coated)

Cat. Num. AAA169144

• Synonyms: Radius 24-Well Cell Migration Assay; (ECM Array Coated); (ECM Array Coated) assay kit

• Preparation and Storage: Upon receipt, aliquot and store the Radius Gel Removal Solution and DAPI Fluorescence Stain at -20 degree C (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4 degree C. All other kit components should be stored at room temperature until the kit's expiration date.

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Related Product Information for Radius 24-Well Cell Migration Assay, (ECM Array Coated) assay kit

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). The Radius Cell Migration Assay Kit utilizes a proprietary 24-well plate, which has been pre-coated with various ECM proteins (see Migration Plate Layout below), to monitor the migratory properties of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot (Radius Gel) where cells cannot attach. When adherent cells are seeded in the Radius Cell Migration well, they attach outside of the Radius Gel coated area. Once firm cell attachment is achieved, the hydrogel is quickly removed to expose a cell-free region to study cell migration/closure. This format provides a robust in vitro system to measure 2-D cell migration, screen potential inhibitors and study cytoskeleton reorganization events. The Radius Cell Migration Assay Kit is designed for High Content Analysis applications or imaging software and is adaptable to liquid handling equipment. Each kit provides sufficient quantities to perform 24 migration tests (18 ECM protein-coated wells, 6 uncoated wells).

Product Categories/Family for Radius 24-Well Cell Migration Assay, (ECM Array Coated) assay kit

Cell Based Assays; Cell Migration; Invasion and Wound Healing; Gap Closure Assays; Cell Migration Assays; 2D Gap Closure

References

1. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. (2003) Science 302, 1704-9.
2. Horwitz R, Webb D. (2003) Curr Biol. 13, R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84, 359-369.