Testing Data
CytoSelect 96-well Cell Invasion, Fluorometric Assay Kit
CytoSelect 96-well Cell Invasion, Fluorometric
Synonyms
CytoSelect 96-well Cell Invasion; Fluorometric; Fluorometric assay kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for CytoSelect 96-well Cell Invasion, Fluorometric assay kit
• Fully quantify cell invasion with no manual cell counting
• Plate inserts are precoated with ECM basement membrane
The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.
Introduction: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased.
MyBioSource's CytoSelect 96-well Cell Invasion Assay Kit utilizes basement membrane-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye.
MyBioSource's CytoSelect 96-well Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. The kit contains sufficient reagents for the evaluation of 96 samples.
• Plate inserts are precoated with ECM basement membrane
The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.
Introduction: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased.
MyBioSource's CytoSelect 96-well Cell Invasion Assay Kit utilizes basement membrane-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye.
MyBioSource's CytoSelect 96-well Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. The kit contains sufficient reagents for the evaluation of 96 samples.
Product Categories/Family for CytoSelect 96-well Cell Invasion, Fluorometric assay kit
References
1. Erkell, L. J., Schirrmacher, V. (1988) Cancer Res 48, 6933-6937.
2. Montgomery, A. M. P., De Clerck, Y. A., Langley, K. E., Reisfeld, R. A., Mueller, B. M. (1993) Cancer Res 53,693-700.
3. Monsky, W. L., Lin, C. Y., Aoyama, A., Kelly, T., Akiyama, S. K., Mueller, S. C., Chen, W. T. (1994) Cancer Res 54,5702-5710.
1. Leung, W.H. et al. (2013). Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXR{gamma} activation. J. Exp. Med. 210:2675-2692.
2. Nakayama, K. et al. (2013). cAMP-response element-binding Protein (CREB) and NF-{kappa}B transcription factors are activated during prolonged hypoxia and cooperatively regulate the induction of matrix metalloproteinase MMP1. J. Biol. Chem. 288:22584-22595.
3. Chen, Z. et al. (2012). The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal transition via up-regulation of N-Myc downstream-regulated gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028.
4. Beach, J.R. et al. (2011). Myosin II isoform switching mediates invasiveness after TGF-beta-induced epithelial-mesenchymal transition. PNAS 108:17991-17996.
5. Eckstein, N. et al. (2009). Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Cancer Res. 69:2996-3003.
6. Lam, K.K.W. et al. (2009). Glycodelin-A as a modulator of trophoblast invasion. Hum. Reprod. 24:2093-2103.
7. Eckstein, N. et al. (2008). EGFR-pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem. 283:739-750.
8. Thal, D.R. et al. (2008). Expression or coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy. J. Pathol. 214:415-424.
9. Neil, J.R. et al. (2008). Cox-2 inactivates smad signaling and enhances EMT stimulated by TGFbeta through a PGE2-dependent mechanism. Carcinogenesis 29:2227-2235.
10. Ji, H. et al. (2007). LKB1 modulates lung cancer differentiation and metastasis. Nature 448:807-810.
2. Montgomery, A. M. P., De Clerck, Y. A., Langley, K. E., Reisfeld, R. A., Mueller, B. M. (1993) Cancer Res 53,693-700.
3. Monsky, W. L., Lin, C. Y., Aoyama, A., Kelly, T., Akiyama, S. K., Mueller, S. C., Chen, W. T. (1994) Cancer Res 54,5702-5710.
1. Leung, W.H. et al. (2013). Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXR{gamma} activation. J. Exp. Med. 210:2675-2692.
2. Nakayama, K. et al. (2013). cAMP-response element-binding Protein (CREB) and NF-{kappa}B transcription factors are activated during prolonged hypoxia and cooperatively regulate the induction of matrix metalloproteinase MMP1. J. Biol. Chem. 288:22584-22595.
3. Chen, Z. et al. (2012). The iron chelators Dp44mT and DFO inhibit TGF-beta-induced epithelial-mesenchymal transition via up-regulation of N-Myc downstream-regulated gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028.
4. Beach, J.R. et al. (2011). Myosin II isoform switching mediates invasiveness after TGF-beta-induced epithelial-mesenchymal transition. PNAS 108:17991-17996.
5. Eckstein, N. et al. (2009). Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Cancer Res. 69:2996-3003.
6. Lam, K.K.W. et al. (2009). Glycodelin-A as a modulator of trophoblast invasion. Hum. Reprod. 24:2093-2103.
7. Eckstein, N. et al. (2008). EGFR-pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem. 283:739-750.
8. Thal, D.R. et al. (2008). Expression or coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy. J. Pathol. 214:415-424.
9. Neil, J.R. et al. (2008). Cox-2 inactivates smad signaling and enhances EMT stimulated by TGFbeta through a PGE2-dependent mechanism. Carcinogenesis 29:2227-2235.
10. Ji, H. et al. (2007). LKB1 modulates lung cancer differentiation and metastasis. Nature 448:807-810.
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Product Notes
The CytoSelect 96-well Cell Invasion, Fluorometric (Catalog #AAA168452) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect 96-well Cell Invasion, Fluorometric, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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