CytoSelect 24-well Cell Migration Assay (3 um), Fluorometric Assay Kit

CytoSelect 24-well Cell Migration Assay (3 um), Fluorometric

Cat. Num. AAA168201

• Synonyms: CytoSelect 24-well Cell Migration Assay (3 um); Fluorometric; Fluorometric assay kit

• Preparation and Storage: Store all components at 4 degree C until their expiration dates.

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Related Product Information for CytoSelect 24-well Cell Migration Assay (3 um), Fluorometric assay kit

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect Cell Migration Assay Kit utilizes polycarbonate membrane inserts (3 mum pore size) to assay the migratory properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory cells (i.e. cotton swabbing). Any migratory cells are first dissociated from the membrane, then lysed and detected by the patented CyQuant GR Dye (Invitrogen). CytoSelect Cell Migration Assay Kit provides a robust system for the quantitative determination of cell migration. The kit contains sufficient reagents for the evaluation of 12 samples. The 3 mum pore size is optimal for leukocyte chemotaxis. However, in the case of epithelial and fibroblast, a larger pore size (8 mum) is recommended. The CytoSelect Cell Migration Assay Kit contains polycarbonate membrane inserts (3 mum pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards chemoattractants (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. These migratory cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).

Product Categories/Family for CytoSelect 24-well Cell Migration Assay (3 um), Fluorometric assay kit

Cell Based Assays; Cell Migration; Invasion and Wound Healing; Boyden Chamber Assays; Chemotaxis Assays

References

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