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Testing Data
CytoSelect 96-well Collagen Assay Kit
CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric
Synonyms
CytoSelect 96-well Collagen; CytoSelect 96-well Collagen Cell Invasion Assay; Fluorometric; CytoSelect 96-well Collagen assay kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
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Related Product Information for CytoSelect 96-well Collagen assay kit
Background: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect 96-well Collagen Cell Invasion Assay Kit utilizes Bovine Type I Collagen-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye. The CytoSelect 96-well Collagen Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. It contains sufficient reagents for the evaluation of 96 samples.
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References
1. Erkell, L. J., Schirrmacher, V. (1988) Cancer Res 48, 6933-6937.
2. Montgomery, A. M. P., De Clerck, Y. A., Langley, K. E., Reisfeld, R. A., Mueller, B. M. (1993) Cancer Res 53,693-700.
3. Monsky, W. L., Lin, C. Y., Aoyama, A., Kelly, T., Akiyama, S. K., Mueller, S. C., Chen, W. T. (1994) Cancer Res 54,5702-5710.
1. Ishiba, T. e. al. (2014). Periostin suppression induces decorin secretion leading to reduced breast cancer cell motility and invasion. Sci Rep. 4:7069.
2. Ziemann, A. et al. (2013). CRN2 enhances the invasiveness of glioblastoma cells. Neuro Oncology 10.1093/neuonc/nos388.
3. Liu, X. et al. (2013). Antiproliferative, antiinvasive, and proapoptotic activity of folate receptor alpha-targeted liposomal doxorubicin in nonfunctional pituitary adenoma cells. Endocrinology 154:1414-1423.
4. Ziemann, A. et al. (2013). CRN2 enhances the invasiveness of glioblastoma cells. Neuro Oncology 10.1093/neuonc/nos388.
2. Montgomery, A. M. P., De Clerck, Y. A., Langley, K. E., Reisfeld, R. A., Mueller, B. M. (1993) Cancer Res 53,693-700.
3. Monsky, W. L., Lin, C. Y., Aoyama, A., Kelly, T., Akiyama, S. K., Mueller, S. C., Chen, W. T. (1994) Cancer Res 54,5702-5710.
1. Ishiba, T. e. al. (2014). Periostin suppression induces decorin secretion leading to reduced breast cancer cell motility and invasion. Sci Rep. 4:7069.
2. Ziemann, A. et al. (2013). CRN2 enhances the invasiveness of glioblastoma cells. Neuro Oncology 10.1093/neuonc/nos388.
3. Liu, X. et al. (2013). Antiproliferative, antiinvasive, and proapoptotic activity of folate receptor alpha-targeted liposomal doxorubicin in nonfunctional pituitary adenoma cells. Endocrinology 154:1414-1423.
4. Ziemann, A. et al. (2013). CRN2 enhances the invasiveness of glioblastoma cells. Neuro Oncology 10.1093/neuonc/nos388.
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Product Notes
The CytoSelect 96-well Collagen (Catalog #AAA168013) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect 96-well Collagen, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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