Human Phospholipase C Zeta 1 (PLCz1) ELISA Kit | PLCz1 elisa kit

Human Phospholipase C Zeta 1 (PLCz1) ELISA Kit

Cat. Num. AAA456333

• Gene Names: PLCZ1; SPGF17; PLCzeta; NYD-SP27; PLC-zeta-1
• Reactivity: Human
• Synonyms: Phospholipase C Zeta 1 (PLCz1); Human Phospholipase C Zeta 1 (PLCz1) ELISA Kit; PLCz1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLCz1. No significant cross-reactivity or interference between PLCz1 and analogues was observed.
• Sequence Length: 504

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLCz1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLCz1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLCz1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLCz1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLCz1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLCz1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLCz1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLCz1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1061899953
• NCBI GeneID: 89869
• NCBI Accession #: NP_001317698.1
• NCBI GenBank Nucleotide #: NM_001330769.1
• UniProt Accession #: Q86YW0; Q08AQ7; Q96J70
• Molecular Weight: 57,804 Da
• NCBI Official Full Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform 3
• NCBI Official Synonym Full Names: phospholipase C zeta 1
• NCBI Official Symbol: PLCZ1
• NCBI Official Synonym Symbols: SPGF17; PLCzeta; NYD-SP27; PLC-zeta-1
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCZ1
• UniProt Synonym Gene Names: PLC-zeta-1

NCBI Description

The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.