Hydrogen Peroxide/Peroxidase Assay Kit
OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Colorimetric)
Synonyms
Hydrogen Peroxide/Peroxidase; OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Colorimetric); Hydrogen Peroxide/Peroxidase assay kit
Preparation and Storage
Upon receipt, aliquot and store the Colorimetric Probe and HRP at -20 degree C
Avoid multiple freeze/thaw cycles
Store the remaining kit components at 4 degree C
The Colorimetric Probe is light sensitive and must be stored accordingly.
Avoid multiple freeze/thaw cycles
Store the remaining kit components at 4 degree C
The Colorimetric Probe is light sensitive and must be stored accordingly.
Related Product Information for Hydrogen Peroxide/Peroxidase assay kit
Principle of the Assay: The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative colorimetric assay for hydrogen peroxide or peroxidase. In the presence of peroxidase, the probe reacts with H2O2 in a 1:1 stoichiometry to produce a bright pink colored product. This product can be easily read by a standard colorimetric microplate reader with a filter in the 540-570 nm range. Absorbance values are proportional to the H2O2 or peroxidase levels within the samples, depending on the assay employed. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
Background/Introduction: Oxidative stress is a physiological condition where there is an imbalance between concentrations of reactive oxygen species (ROS) and antioxidants. Research has shown that excessive ROS accumulation will lead to cellular injury, such as damage to DNA, proteins, and lipid membranes. Peroxides, such as hydrogen peroxide (H2O2), are some of the most well documented ROS produced under oxidative stress conditions. Hydrogen peroxide is an ROS that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. Hydrogen peroxide is poisonous to eukaryotic cells and in high doses can initiate oxidation of DNA, lipids, and proteins, which can lead to mutagenesis and cell death. The cellular damage caused by peroxides have been implicated in the development of many pathological conditions, such as ageing, asthma, arthritis, diabetes, cardiovascular disease, atherosclerosis, Down's Syndrome, and neurodegenerative diseases. The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a simple HTS-compatible assay for measuring hydrogen peroxide concentrations or peroxidase activities in biological samples without any need for pretreatment. The colorimetric probe reacts with H2O2 and horseradish peroxidase enzyme (HRP) to produce a pink colored product. The probe has less background and greater stability than the commonly used Xylenol Orange (FOX) colorimetric assay for H2O2. The probe can be also used as an ultrasensitive assay for peroxidase activity when H2O2 is in excess. The kit has a detection sensitivity limit of 0.8 uM (H2O2) or 0.16 mU/mL (peroxidase). Each kit provides sufficient reagents to perform up to 500 assays, including standard curve and unknown samples.
Background/Introduction: Oxidative stress is a physiological condition where there is an imbalance between concentrations of reactive oxygen species (ROS) and antioxidants. Research has shown that excessive ROS accumulation will lead to cellular injury, such as damage to DNA, proteins, and lipid membranes. Peroxides, such as hydrogen peroxide (H2O2), are some of the most well documented ROS produced under oxidative stress conditions. Hydrogen peroxide is an ROS that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. Hydrogen peroxide is poisonous to eukaryotic cells and in high doses can initiate oxidation of DNA, lipids, and proteins, which can lead to mutagenesis and cell death. The cellular damage caused by peroxides have been implicated in the development of many pathological conditions, such as ageing, asthma, arthritis, diabetes, cardiovascular disease, atherosclerosis, Down's Syndrome, and neurodegenerative diseases. The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a simple HTS-compatible assay for measuring hydrogen peroxide concentrations or peroxidase activities in biological samples without any need for pretreatment. The colorimetric probe reacts with H2O2 and horseradish peroxidase enzyme (HRP) to produce a pink colored product. The probe has less background and greater stability than the commonly used Xylenol Orange (FOX) colorimetric assay for H2O2. The probe can be also used as an ultrasensitive assay for peroxidase activity when H2O2 is in excess. The kit has a detection sensitivity limit of 0.8 uM (H2O2) or 0.16 mU/mL (peroxidase). Each kit provides sufficient reagents to perform up to 500 assays, including standard curve and unknown samples.
Product Categories/Family for Hydrogen Peroxide/Peroxidase assay kit
Similar Products
Product Notes
The Hydrogen Peroxide/Peroxidase (Catalog #AAA169261) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hydrogen Peroxide/Peroxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
