Rabbit RAE1 Polyclonal Antibody | anti-RAE1 antibody

RAE1 Polyclonal Antibody

Cat. Num. AAA3011687

• Gene Names: RAE1; Gle2; MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAE1; Polyclonal Antibody; RAE1 Polyclonal Antibody; mRNA export factor; Rae1 protein homolog; mRNA-associated protein mrnp 41; MRNP41; anti-RAE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAE1 polyclonal antibody detects endogenous levels of RAE1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 368

• Applicable Applications for anti-RAE1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human RAE1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAE1 polyclonal antibody)

Related Product Information for anti-RAE1 antibody

Natural killer (NK) cells attack tumor and infected cells, but the receptors and ligands that stimulate them are poorly understood. Two murine ligands for the lectin-like receptor NKG2D, H60 and retinoic acid early inducible (Rae- 1alpha, beta, gamma, delta and epsilon), are distant relatives of major histocompatibility complex class I molecules. These molecules are encoded by Rae-1 and H60 minor histocompatibility antigen genes on mouse chromosome 10 and show weak homology with MHC class I. Expression of the NKG2D ligands is low or absent on normal, adult tissues; however, they are constitutively expressed on some tumors and upregulated by retinoic acid. Ectopic expression of Rae-1 and H60 confers target susceptibility to NK cell attack. NKG2D binds to H60 with approximately 25-fold higher affinity than to Rae-1. Rae-1 and H60 compete directly for occupancy of NKG2D; therefore, NKG2D can be occupied by only one ligand at a time. Additionally, Rae-1 and H60 ligands of the NKG2D receptor stimulate tumor immunity.

NCBI and Uniprot Product Information

• NCBI GI #: 4506399
• NCBI GeneID: 8480
• NCBI Accession #: NP_003601.1
• NCBI GenBank Nucleotide #: NP_003601.1
• UniProt Accession #: P78406
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: mRNA export factor
• NCBI Official Synonym Full Names: ribonucleic acid export 1
• NCBI Official Symbol: RAE1
• NCBI Official Synonym Symbols: Gle2; MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
• NCBI Protein Information: mRNA export factor
• UniProt Protein Name: mRNA export factor
• Protein Family: Protein
• UniProt Gene Name: RAE1
• UniProt Synonym Gene Names: MRNP41
• UniProt Entry Name: RAE1L_HUMAN

NCBI Description

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RAE1: Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Belongs to the WD repeat rae1 family.

Protein type: Motility/polarity/chemotaxis; RNA-binding

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: nuclear outer membrane; cytoskeleton; cytoplasm; nucleolus; nuclear pore; nuclear envelope; nucleus

Molecular Function: RNA binding; microtubule binding

Biological Process: mRNA export from nucleus; viral reproduction; hexose transport; cytokine and chemokine mediated signaling pathway; carbohydrate metabolic process; mitotic nuclear envelope disassembly; pathogenesis; viral transcription; gene expression; mitotic cell cycle; glucose transport; viral infectious cycle; transmembrane transport