Guinea Pig GATA Binding Protein 2 ELISA Kit | GATA2 elisa kit

Guinea pig GATA Binding Protein 2 ELISA Kit

Cat. Num. AAA720708

• Gene Names: GATA2; DCML; IMD21; NFE1B; MONOMAC
• Reactivity: Guinea Pig
• Synonyms: GATA Binding Protein 2; Guinea pig GATA Binding Protein 2 ELISA Kit; GATA2 elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GATA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig GATA2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA2 elisa kit

Principle of the assay: GATA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GATA2 antibody and an GATA2-HRP conjugate. The assay sample and buffer are incubated together with GATA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA2 concentration since GATA2 from samples and GATA2-HRP conjugate compete for the anti-GATA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GATA2 from the sample, fewer sites are left to bind GATA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GATA2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 30410878
• NCBI GeneID: 2624
• Molecular Weight: 49,106 Da
• NCBI Official Full Name: GATA2 protein
• NCBI Official Synonym Full Names: GATA binding protein 2
• NCBI Official Symbol: GATA2
• NCBI Official Synonym Symbols: DCML; IMD21; NFE1B; MONOMAC
• NCBI Protein Information: endothelial transcription factor GATA-2
• UniProt Protein Name: Endothelial transcription factor GATA-2
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA2
• UniProt Entry Name: GATA2_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; protein complex

Molecular Function: protein binding; zinc ion binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: embryonic placenta development; inner ear morphogenesis; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of erythrocyte differentiation; cell maturation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; negative regulation of fat cell differentiation; phagocytosis; cell differentiation in hindbrain; positive regulation of angiogenesis; negative regulation of macrophage differentiation; pituitary gland development; ventral spinal cord interneuron differentiation; commitment of a neuronal cell to a specific type of neuron in the forebrain; homeostasis of number of cells within a tissue; positive regulation of phagocytosis; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; blood coagulation; urogenital system development; central nervous system neuron development

Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome