Typical Standard Curve/Testing Data
15-PGDH assay kit
15-PGDH Inhibitor Screening Kit (Fluorometric)
Synonyms
15-PGDH; 15-PGDH Inhibitor Screening Kit (Fluorometric); Prostaglandin-Endoperoxide Synthase 2 Inhibitor Screening Kit (Fluorometric); PTGS2 Inhibitor Screening Kit (Fluorometric); 15-PGDH assay kit
Application Notes
Screening/studying/characterizing 15-PGDH inhibitors.
Preparation and Storage
Store at -20 degree C for 12 months.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for 15-PGDH assay kit
Background/Introduction: 15-hydroxyprostaglandin dehydrogenase (15-PGDH, EC: 1.1.1.141) catalyzes the oxidation of Prostaglandins (PG) to 15-keto metabolites which show greatly reduced biological activity when compared to Prostaglandins. 15-PGDH catalyzes the rate-limiting step of PG catabolism. More recently, the inhibition of 15-PGDH has been reported as a negative regulator of tissue repair and inhibition of this enzyme can potentiate tissue regeneration in mouse models. Thus, pharmacological inhibition of 15-PGDH could have clinical implications by accelerating potential tissue regeneration after damage. In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing NAD. NADH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=535/587 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence.15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.
Product Categories/Family for 15-PGDH assay kit
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Product Notes
The 15-PGDH (Catalog #AAA846792) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screening/studying/characterizing 15-PGDH inhibitors. Researchers should empirically determine the suitability of the 15-PGDH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "15-PGDH, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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