Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
USP35 recombinant protein
USP35, Active
Applications
Western Blot
Synonyms
USP35; Active; KIAA1372; USP35 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for USP35 recombinant protein
Activity Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for USP35 recombinant protein
Recombinant human USP35 (395-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The USP35 gene accession number is NM_020798.
USP35 (ubiquitin carboxyl-terminal hydrolase 35) is a member of the peptidase C19 family of ubiquitin-specific proteases that plays a role in the regulation of mitosis. USP35 deubiquitinates Aurora B, which promotes stability and activates Aurora B. The reaction also antagonizes the APCCDH1-dependent K11-linked ubiquitination of Aurora B. USP35 expression is regulated by FoxM1 as well as Aurora B in the cell cycle (1). USP35 induced by miR let-7a can inhibit cell proliferation by inhibiting NF-kappaB activation via deubiquitination and stabilization of ABIN-2 protein (2). The miR let-7a-USP35-ABIN-2 pathway can be used as a target for novel cancer therapy.
USP35 (ubiquitin carboxyl-terminal hydrolase 35) is a member of the peptidase C19 family of ubiquitin-specific proteases that plays a role in the regulation of mitosis. USP35 deubiquitinates Aurora B, which promotes stability and activates Aurora B. The reaction also antagonizes the APCCDH1-dependent K11-linked ubiquitination of Aurora B. USP35 expression is regulated by FoxM1 as well as Aurora B in the cell cycle (1). USP35 induced by miR let-7a can inhibit cell proliferation by inhibiting NF-kappaB activation via deubiquitination and stabilization of ABIN-2 protein (2). The miR let-7a-USP35-ABIN-2 pathway can be used as a target for novel cancer therapy.
Product Categories/Family for USP35 recombinant protein
References
1. Park, J.Y. et al: USP35 regulates mitotic progression by modulating the stability of Aurora B. Nature Communications 9: 688, 2018.
2. Liu, C. et al: USP35 activated by miR let-7a inhibits cell proliferation and NF-kappaB activation through stabilization of ABIN-2. Oncotarget 6(29): 27891-906, 2015.
2. Liu, C. et al: USP35 activated by miR let-7a inhibits cell proliferation and NF-kappaB activation through stabilization of ABIN-2. Oncotarget 6(29): 27891-906, 2015.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105kDa
Protein Family
Similar Products
Product Notes
The USP35 (Catalog #AAA517774) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's USP35 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Western Blot (WB). Researchers should empirically determine the suitability of the USP35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP35, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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