Mouse Aminopeptidase Q ELISA Kit | Aqpep elisa kit

Mouse Aminopeptidase Q ELISA Kit

Cat. Num. AAA288258

• Reactivity: Mouse
• Synonyms: Aminopeptidase Q; Mouse Aminopeptidase Q ELISA Kit; Aqpep elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse AP-Q. No significant cross-reactivity or interference was observed.
• Sequence Length: 990

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.078 ng/mL
• Intra-Assay CV: <=6.8%
• Inter-Assay CV: <=9.3%
• Recovery: 92%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aqpep elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AP-Q. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AP-Q and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AP-Q, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AP-Q in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110810408
• UniProt Accession #: Q2KHK3; Q9D633
• Molecular Weight: 63,338 Da
• NCBI Official Full Name: Aminopeptidase Q
• UniProt Protein Name: Aminopeptidase Q
• Protein Family: Aminopeptidase
• UniProt Gene Name: Lvrn
• UniProt Synonym Gene Names: AP-QCurated; APQ
• UniProt Entry Name: AMPQ_MOUSE

Uniprot Description

LVRN: Metalloprotease which may be important for placentation by regulating biological activity of key peptides at the embryo- maternal interface. On synthetic substrates it shows a marked preference for Leu-4-methylcoumaryl-7-amide (Leu-MCA) over Met- MCA, Arg-LCA and Lys-LCA. Cleaves the N-terminal amino acid of several peptides such as angiotensin-3, kisspeptin-10 and endokinin C. Belongs to the peptidase M1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.-.-; Membrane protein, integral; EC 3.4.11.-

Cellular Component: cytoplasm; integral to membrane; membrane; plasma membrane

Molecular Function: hydrolase activity; metal ion binding; metallopeptidase activity; peptidase activity; peptide binding; zinc ion binding

Biological Process: cell-cell signaling; peptide catabolic process; proteolysis; regulation of blood pressure; signal transduction