anti-Human FA7 Antibody | anti-FA7 antibody

FA7 (light chain, Cleaved-Arg212) Antibody

Cat. Num. AAA853920

• Gene Names: F7; SPCA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: FA7; FA7 (light chain; Cleaved-Arg212) Antibody; Coagulation factor VII [Precursor]; EC 3.4.21.21; Serum prothrombin conversion accelerator; SPCA; Proconvertin; Eptacog alfa; Factor VII light chain; Factor VII heavy chain; F7; anti-FA7 antibody

• Reactivity: Human
• Specificity: FA7 (light chain, Cleaved-Arg212) antibody detects endogenous levels of fragment of activated FA7 resulting from cleavage adjacent to Arg212.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 466

• Applicable Applications for anti-FA7 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human FA7.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Cl-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FA7 (light chain, Cleaved-Arg212) antibody. Western blot analysis of extracts from Jurkat cells, treated with eto (25uM, 24hours), using FA7 (light chain, Cleaved-Arg212) antibody.)

References

Hagen F.S., Proc. Natl. Acad. Sci. U.S.A. 83:2412-2416(1986). O'Hara P.J., Proc. Natl. Acad. Sci. U.S.A. 84:5158-5162(1987). Sabater-Lleal M., Hum. Genet. 118:741-751(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4503645
• NCBI GeneID: 2155
• NCBI Accession #: NP_000122.1
• NCBI GenBank Nucleotide #: NM_000131.4
• UniProt Accession #: P08709; Q14339; Q5JVF1; Q5JVF2; Q9UD52; Q9UD53; Q9UD54; B0YJC8
• Molecular Weight: 49,320 Da
• NCBI Official Full Name: coagulation factor VII isoform a preproprotein
• NCBI Official Synonym Full Names: coagulation factor VII
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: SPCA
• NCBI Protein Information: coagulation factor VII
• UniProt Protein Name: Coagulation factor VII
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: SPCA

NCBI Description

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.