Typical Testing Data/Standard Curve (for reference only)
Mouse Transketolase ELISA Kit | Tkt elisa kit
Mouse Transketolase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that binds magnesium and thiamine pyrophosphate and catalyzes the transfer of sugar phosphates to an aldose acceptor. This enzyme is a key component of the pentose phosphate pathway during glycolysis. It is significantly expressed in the cornea and may be involved in the cellular response against oxidative stress. Haploinsufficiency of this gene leads to decreased growth and reduction of adipose tissue. [provided by RefSeq, Dec 2013]
Uniprot Description
TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family.
Protein type: EC 2.2.1.1; Carbohydrate Metabolism - pentose phosphate pathway; Transferase
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; myelin sheath; nucleoplasm; nucleus; peroxisome; vesicle
Molecular Function: carbohydrate binding; catalytic activity; cofactor binding; magnesium ion binding; metal ion binding; monosaccharide binding; protein homodimerization activity; thiamin pyrophosphate binding; transferase activity; transketolase activity
Biological Process: glyceraldehyde-3-phosphate biosynthetic process; metabolic process; pentose-phosphate shunt; pentose-phosphate shunt, non-oxidative branch; regulation of growth; ribose phosphate biosynthetic process
Research Articles on Tkt
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Product Notes
The Mouse Tkt tkt (Catalog #AAA2881863) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881863 ELISA Kit recognizes Mouse Tkt. It is sometimes possible for the material contained within the vial of "Transketolase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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