Typical Testing Data/Standard Curve (for reference only)
Rat Catechol O-methyltransferase ELISA Kit | Comt elisa kit
Rat Catechol O-methyltransferase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catechol O-methyltransferase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forCatechol O-methyltransferase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Catechol O-methyltransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Catechol O-methyltransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Membrane protein, integral; Amino Acid Metabolism - tyrosine; EC 2.1.1.6; Methyltransferase
Cellular Component: axon; cytosol; dendrite; dendritic spine; integral to membrane; membrane; mitochondrion; plasma membrane; postsynaptic membrane
Molecular Function: catechol O-methyltransferase activity; magnesium ion binding
Biological Process: catechol metabolic process; cellular response to phosphate starvation; developmental process; dopamine catabolic process; dopamine metabolic process; estrogen metabolic process; female pregnancy; learning; methylation; negative regulation of dopamine metabolic process; negative regulation of smooth muscle cell proliferation; neurotransmitter catabolic process; positive regulation of homocysteine metabolic process; regulation of sensory perception of pain; reproductive process in a multicellular organism; response to drug; response to lipopolysaccharide; response to organic cyclic substance; response to pain; S-adenosylhomocysteine metabolic process; S-adenosylmethionine metabolic process; short-term memory
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Product Notes
The Rat Comt comt (Catalog #AAA2880296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880296 ELISA Kit recognizes Rat Comt. It is sometimes possible for the material contained within the vial of "Catechol O-methyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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