Human Aminopeptidase Q ELISA Kit | AQPEP elisa kit

Human Aminopeptidase Q ELISA Kit

Cat. Num. AAA762982

• Gene Names: LVRN; APQ; AQPEP; TAQPEP
• Reactivity: Human
• Synonyms: Aminopeptidase Q; Human Aminopeptidase Q ELISA Kit; AQPEP/AP-Q/Aminopeptidase Q/LVRN/CHL2 antigen/Laeverin; AQPEP elisa kit

• Reactivity: Human
• Sequence Length: 990

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AQPEP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AQPEP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AQPEP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AQPEP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AQPEP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 194239713
• NCBI GeneID: 206338
• NCBI Accession #: NP_776161.3
• NCBI GenBank Nucleotide #: NM_173800.4
• Molecular Weight: 23,178 Da
• NCBI Official Full Name: aminopeptidase Q
• NCBI Official Synonym Full Names: laeverin
• NCBI Official Symbol: LVRN
• NCBI Official Synonym Symbols: APQ; AQPEP; TAQPEP
• NCBI Protein Information: aminopeptidase Q
• UniProt Protein Name: Aminopeptidase Q
• Protein Family: Aminopeptidase
• UniProt Gene Name: LVRN
• UniProt Synonym Gene Names: AP-QCurated; APQ
• UniProt Entry Name: AMPQ_HUMAN

Uniprot Description

LVRN: Metalloprotease which may be important for placentation by regulating biological activity of key peptides at the embryo- maternal interface. On synthetic substrates it shows a marked preference for Leu-4-methylcoumaryl-7-amide (Leu-MCA) over Met- MCA, Arg-LCA and Lys-LCA. Cleaves the N-terminal amino acid of several peptides such as angiotensin-3, kisspeptin-10 and endokinin C. Belongs to the peptidase M1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.-.-; Membrane protein, integral; EC 3.4.11.-; Protease

Chromosomal Location of Human Ortholog: 5q23.1

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: peptide binding; zinc ion binding

Biological Process: cell-cell signaling; peptide catabolic process; proteolysis; regulation of blood pressure; signal transduction