Rat Bcl-2-binding component 3 ELISA Kit | Bbc3 elisa kit

Rat Bcl-2-binding component 3 ELISA Kit

Cat. Num. AAA288278

• Gene Names: Bbc3; Puma
• Reactivity: Rat
• Synonyms: Bcl-2-binding component 3; Rat Bcl-2-binding component 3 ELISA Kit; Bbc3 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Bcl-2-binding component 3. No significant cross-reactivity or interference was observed.
• Sequence Length: 193

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.091 ng/mL
• Intra-Assay CV: <=4.4%
• Inter-Assay CV: <=7.1%
• Recovery: 92%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bbc3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl-2-binding component 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bcl-2-binding component 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bcl-2-binding component 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Bcl-2-binding component 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27753966
• NCBI GeneID: 317673
• NCBI Accession #: NP_776209.1
• NCBI GenBank Nucleotide #: NM_173837.2
• UniProt Accession #: Q80ZG6
• Molecular Weight: 20,718 Da
• NCBI Official Full Name: bcl-2-binding component 3
• NCBI Official Synonym Full Names: Bcl-2 binding component 3
• NCBI Official Symbol: Bbc3
• NCBI Official Synonym Symbols: Puma
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: Bbc3
• UniProt Synonym Gene Names: Puma
• UniProt Entry Name: BBC3_RAT

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: endoplasmic reticulum; lysosome; mitochondrion

Molecular Function: ATPase binding; glycoprotein binding; protein binding

Biological Process: apoptosis; brain development; caspase activation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; glial cell differentiation; negative regulation of growth; positive regulation of apoptosis; positive regulation of neuron apoptosis; positive regulation of protein homooligomerization; reduction of endoplasmic reticulum calcium ion concentration; release of cytochrome c from mitochondria; release of sequestered calcium ion into cytosol; response to DNA damage stimulus