Rat Apolipoprotein C1 ELISA Kit | ApoC1 elisa kit

Rat ApoC1 (Apolipoprotein C1) ELISA Kit

Cat. Num. AAA2533550

• Gene Names: APOC1; Apo-CI; ApoC-I
• Reactivity: Rat
• Synonyms: Apolipoprotein C1; Rat ApoC1 (Apolipoprotein C1) ELISA Kit; ApoC1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatApoC1. No significant cross-reactivity or interference between RatApoC1 and analogues was observed.
• Sequence Length: 83

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500ng/mL
• Sensitivity: Min: 4.688ng/mL ; Max: 500ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ApoC1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatApoC1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoC1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoC1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoC1.You can calculate the concentration of ApoC1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502157
• NCBI GeneID: 341
• NCBI Accession #: NP_001636.1
• NCBI GenBank Nucleotide #: NM_001645.3
• Molecular Weight: 9,332 Da
• NCBI Official Full Name: apolipoprotein C-I
• NCBI Official Synonym Full Names: apolipoprotein C-I
• NCBI Official Symbol: APOC1
• NCBI Official Synonym Symbols: Apo-CI; ApoC-I
• NCBI Protein Information: apolipoprotein C-I
• UniProt Protein Name: Apolipoprotein C-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC1
• UniProt Synonym Gene Names: Apo-CI; ApoC-I
• UniProt Entry Name: APOC1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family.

Protein type: Secreted; Inhibitor; Endoplasmic reticulum; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: chylomicron; endoplasmic reticulum

Molecular Function: fatty acid binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipase inhibitor activity

Biological Process: cholesterol efflux; cholesterol metabolic process; lipid metabolic process; lipoprotein metabolic process; negative regulation of cholesterol transport; negative regulation of fatty acid biosynthetic process; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; phospholipid efflux; positive regulation of catalytic activity; regulation of cholesterol transport; triacylglycerol metabolic process