PILRB recombinant protein

PILRB Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121522

• Gene Names: PILRB; FDFACT1; FDFACT2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PILRB; PILRB Protein; Human; Recombinant (His Tag); Human PILRB Protein (His Tag); FDFACT1 Protein; FDFACT2 Protein; paired immunoglobin-like type 2 receptor beta; PILRB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln20-Ala189

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PILRB (NP_839956.1)(Gln20-Ala189) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

Wilson MD, et al. (2006) Comparative analysis of the paired immunoglobulin-like receptor (PILR) locus in six mammalian genomes: duplication, conversion, and the birth of new genes. Physiol Genomics. 27 (3): 201-18.Orr MT, et al. (2010) Natural killer cell education and tolerance. Cell. 142(6): 847-56.Mousseau DD, et al. (2000) PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. J Biol Chem. 275 (6): 4467-74.

NCBI and Uniprot Product Information

• NCBI GI #: 30179911
• NCBI GeneID: 29990
• NCBI Accession #: NP_839956.1
• NCBI GenBank Nucleotide #: NM_178238.3
• UniProt Accession #: Q9UKJ0; Q69YF9; Q9HBS0
• Molecular Weight: 19.6kD
• NCBI Official Full Name: paired immunoglobulin-like type 2 receptor beta
• NCBI Official Synonym Full Names: paired immunoglobin-like type 2 receptor beta
• NCBI Official Symbol: PILRB
• NCBI Official Synonym Symbols: FDFACT1; FDFACT2
• NCBI Protein Information: paired immunoglobulin-like type 2 receptor beta; activating receptor PILRbeta; cell surface receptor FDFACT; activating receptor PILR-beta; cell surface receptor FDFACT1; cell surface receptor FDFACT2; paired immunoglobin-like receptor beta; paired immuno
• UniProt Protein Name: Paired immunoglobulin-like type 2 receptor beta
• Protein Family: Paired immunoglobulin-like type 2 receptor
• UniProt Gene Name: PILRB
• UniProt Synonym Gene Names: FDFACT
• UniProt Entry Name: PILRB_HUMAN

NCBI Description

The paired immunoglobin-like type 2 receptors consist of highly related activating and inhibitory receptors that are involved in the regulation of many aspects of the immune system. The paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This gene encodes the activating member of the receptor pair and contains a truncated cytoplasmic tail relative to its inhibitory counterpart (PILRA), that has a long cytoplasmic tail with immunoreceptor tyrosine-based inhibitory (ITIM) motifs. This gene is thought to have arisen from a duplication of the inhibitory PILRA gene and evolved to acquire its activating function. [provided by RefSeq, Jun 2013]

Uniprot Description

PILRB: Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRB is thought to act as a cellular signaling activating receptor that associates with ITAM-bearing adapter molecules on the cell surface. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: integral to plasma membrane

Molecular Function: protein binding

Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); transmembrane receptor protein tyrosine kinase signaling pathway