Canine Apolipoprotein C1 ELISA Kit | APOC1 elisa kit

Canine Apolipoprotein C1 ELISA Kit

Cat. Num. AAA734854

• Reactivity: Canine
• Synonyms: Apolipoprotein C1; Canine Apolipoprotein C1 ELISA Kit; APOC1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This APOC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine APOC1. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY APOC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APOC1 antibody and an APOC1-HRP conjugate. The assay sample and buffer are incubated together with APOC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOC1 concentration since APOC1 from samples and APOC1-HRP conjugate compete for the anti-APOC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOC1 from the sample, fewer sites are left to bind APOC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOC1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4502157
• NCBI GeneID: 341
• NCBI Accession #: NP_001636.1
• NCBI GenBank Nucleotide #: NM_001645.3
• Molecular Weight: 9,332 Da
• NCBI Official Full Name: apolipoprotein C-I
• NCBI Official Synonym Full Names: apolipoprotein C-I
• NCBI Official Symbol: APOC1
• NCBI Protein Information: apolipoprotein C-I; apo-CIB; apoC-IB; apolipoprotein C1; apolipoprotein C-I variant I
• UniProt Protein Name: Apolipoprotein C-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC1
• UniProt Entry Name: APOC1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide; Lipid-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: chylomicron; endoplasmic reticulum

Molecular Function: phospholipase inhibitor activity; lipase inhibitor activity; phosphatidylcholine binding; fatty acid binding

Biological Process: positive regulation of catalytic activity; cholesterol metabolic process; negative regulation of cholesterol transport; lipoprotein metabolic process; cholesterol efflux; triacylglycerol metabolic process; negative regulation of lipid catabolic process; phospholipid efflux; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; lipid metabolic process; regulation of cholesterol transport; negative regulation of lipid metabolic process; negative regulation of fatty acid biosynthetic process