Human Apolipoprotein C1 ELISA Kit | APOC1 elisa kit

Human Apolipoprotein C1 ELISA Kit

Cat. Num. AAA720844

• Reactivity: Human
• Synonyms: Apolipoprotein C1; Human Apolipoprotein C1 ELISA Kit; APOC1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC1 elisa kit

Intended Uses: This ApoC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ApoC1. This ELISA kit for research use only!

Principle of the Assay: ApoC1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoC1. Standards or samples are then added to the microtiter plate wells and ApoC1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoC1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoC1 are added to each well to "sandwich" the ApoC1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoC1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOC1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4502157
• NCBI GeneID: 341
• NCBI Accession #: NP_001636.1
• NCBI GenBank Nucleotide #: NM_001645.3
• Molecular Weight: 9,332 Da
• NCBI Official Full Name: apolipoprotein C-I
• NCBI Official Synonym Full Names: apolipoprotein C-I
• NCBI Official Symbol: APOC1
• NCBI Protein Information: apolipoprotein C-I; apo-CIB; apoC-IB; apolipoprotein C1; apolipoprotein C-I variant I
• UniProt Protein Name: Apolipoprotein C-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC1
• UniProt Entry Name: APOC1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family.

Protein type: Endoplasmic reticulum; Secreted, signal peptide; Inhibitor; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: chylomicron; endoplasmic reticulum

Molecular Function: phospholipase inhibitor activity; lipase inhibitor activity; phosphatidylcholine binding; fatty acid binding

Biological Process: positive regulation of catalytic activity; cholesterol metabolic process; negative regulation of cholesterol transport; cholesterol efflux; lipoprotein metabolic process; triacylglycerol metabolic process; negative regulation of lipid catabolic process; phospholipid efflux; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; lipid metabolic process; negative regulation of lipid metabolic process; regulation of cholesterol transport; negative regulation of fatty acid biosynthetic process