Human Apolipoprotein C-I ELISA Kit | APOC1 elisa kit

Human Apolipoprotein C-I ELISA Kit

Cat. Num. AAA2886283

• Gene Names: APOC1; Apo-CI; ApoC-I
• Reactivity: Human
• Synonyms: Apolipoprotein C-I; Human Apolipoprotein C-I ELISA Kit; Apo-CIB; ApoC-IB; Apolipoprotein C1; APOC1; APOC1B; APOC1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Apolipoprotein C-I,Apo-CIB
• Sequence Length: 83

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 1.56 ng/mL - 100 ng/mL
• Sensitivity: < 0.57 ng/mL
• Intra-assay Precision: <=5.1%
• Inter-assay Precision: <=10.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502157
• NCBI GeneID: 341
• NCBI Accession #: NP_001636.1
• NCBI GenBank Nucleotide #: NM_001645.3
• UniProt Accession #: P02654; Q6IB97; B2R526
• Molecular Weight: 9,332 Da
• NCBI Official Full Name: apolipoprotein C-I
• NCBI Official Synonym Full Names: apolipoprotein C-I
• NCBI Official Symbol: APOC1
• NCBI Official Synonym Symbols: Apo-CI; ApoC-I
• NCBI Protein Information: apolipoprotein C-I
• UniProt Protein Name: Apolipoprotein C-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC1
• UniProt Synonym Gene Names: Apo-CI; ApoC-I
• UniProt Entry Name: APOC1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family.

Protein type: Secreted; Secreted, signal peptide; Endoplasmic reticulum; Lipid-binding; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: chylomicron; endoplasmic reticulum

Molecular Function: fatty acid binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipase inhibitor activity

Biological Process: cholesterol efflux; cholesterol metabolic process; lipid metabolic process; lipoprotein metabolic process; negative regulation of cholesterol transport; negative regulation of fatty acid biosynthetic process; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; phospholipid efflux; positive regulation of catalytic activity; regulation of cholesterol transport; triacylglycerol metabolic process