Mouse Apolipoprotein B-100 ELISA Kit | Apob elisa kit

Mouse Apolipoprotein B-100 ELISA Kit

Cat. Num. AAA2886104

• Gene Names: Apob; apob-48; AI315052; apob-100; Apo B-100
• Reactivity: Mouse
• Synonyms: Apolipoprotein B-100; Mouse Apolipoprotein B-100 ELISA Kit; Apo B-100; Apob; Apob elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural Mouse Apo B-100. No significant cross-reactivity or interference was observed.
• Sequence Length: 4505

• Assay Type: Sandwich
• Detection Range: 31.2-2000 pg/mL
• Sensitivity: 15.78 pg/mL
• Intra-Assay CV: <=7.9%
• Inter-Assay CV: <=10.1%
• Recovery: 102%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apob elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of Mouse apolipoprotein b-100,Apo B-100 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo B-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo B-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo B-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Apo B-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161702988
• NCBI GeneID: 238055
• NCBI Accession #: NP_033823.2
• NCBI GenBank Nucleotide #: NM_009693.2
• UniProt Accession #: E9Q414; Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8
• Molecular Weight: 509,432 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: apob-48; AI315052; apob-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_MOUSE

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted; Secreted, signal peptide; Carrier

Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; vesicle membrane

Molecular Function: cholesterol transporter activity; heparin binding; lipid binding; lipid transporter activity; low-density lipoprotein receptor binding; phospholipid binding

Biological Process: artery morphogenesis; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fertilization; in utero embryonic development; lipid catabolic process; lipid metabolic process; lipid transport; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; post-embryonic development; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; sperm motility; spermatogenesis; steroid metabolic process; transport; triacylglycerol catabolic process; triacylglycerol mobilization