Western Blot (WB)
(PMPCA Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PMPCA antibody detected the PMPCA protein (arrow).)
Rabbit anti-Human PMPCA Polyclonal Antibody | anti-PMPCA antibody
PMPCA Antibody (C-term)
Gene Names
PMPCA; P-55; INPP5E; Alpha-MPP
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PMPCA; Polyclonal Antibody; PMPCA Antibody (C-term); Mitochondrial-processing peptidase subunit alpha; Alpha-MPP; P-55; INPP5E; KIAA0123; MPPA; anti-PMPCA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PMPCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-483 amino acids from the C-terminal region of human PMPCA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
454-483
Sequence Length
394
Applicable Applications for anti-PMPCA antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PMPCA Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PMPCA antibody detected the PMPCA protein (arrow).)
Western Blot (WB)
(PMPCA Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PMPCA antibody detected the PMPCA protein (arrow).)
Flow Cytometry (FC/FACS)
(PMPCA Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PMPCA Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PMPCA antibody
References
Hassel, S., et al. Proteomics 4(5):1346-1358(2004)
Harris, R.A., et al. Proteomics 2(2):212-223(2002)
Nagao, Y., et al. J. Biol. Chem. 275(44):34552-34556(2000)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Luciano, P., et al. J. Mol. Biol. 272(2):213-225(1997)
Harris, R.A., et al. Proteomics 2(2):212-223(2002)
Nagao, Y., et al. J. Biol. Chem. 275(44):34552-34556(2000)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Luciano, P., et al. J. Mol. Biol. 272(2):213-225(1997)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58253
NCBI Official Full Name
mitochondrial-processing peptidase subunit alpha isoform 1
NCBI Official Synonym Full Names
peptidase (mitochondrial processing) alpha
NCBI Official Symbol
PMPCA
NCBI Official Synonym Symbols
P-55; INPP5E; Alpha-MPP
NCBI Protein Information
mitochondrial-processing peptidase subunit alpha
UniProt Protein Name
Mitochondrial-processing peptidase subunit alpha
Protein Family
UniProt Gene Name
PMPCA
UniProt Synonym Gene Names
INPP5E; KIAA0123; MPPA
UniProt Entry Name
MPPA_HUMAN
Uniprot Description
PMPCA: Cleaves presequences (transit peptides) from mitochondrial protein precursors. Belongs to the peptidase M16 family.
Protein type: EC 3.4.24.64; Protease; Mitochondrial
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: extracellular space; mitochondrion; mitochondrial matrix; mitochondrial inner membrane
Molecular Function: metal ion binding; metalloendopeptidase activity
Biological Process: cellular protein metabolic process; proteolysis; protein targeting to mitochondrion
Research Articles on PMPCA
Similar Products
Product Notes
The PMPCA pmpca (Catalog #AAA9208337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 454-483. The PMPCA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PMPCA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PMPCA pmpca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMPCA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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