Rabbit anti-Mouse Apolipoprotein B (APOB) Polyclonal Antibody | anti-APOB antibody

Polyclonal Antibody to Apolipoprotein B (APOB)

Cat. Num. AAA2112198

• Gene Names: Apob; apob-48; AI315052; apob-100; Apo B-100
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Apolipoprotein B (APOB); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein B (APOB); Apo-B; FLDB; Apo B-100; Apo B-48; anti-APOB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 500ug/ml (varies by lot)
• Sequence: Leu32~Met306

• Applicable Applications for anti-APOB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-2ug/mL; IHC: 5-20ug/mL; ICC: 5-20ug/mL
• Immunogen Sequence: Leu32~Met306
• Cross Reactivity: Mouse
• Conjugation: None
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Lane1: Mouse Serum; Lane2: Mouse Liver lysate Primary Ab: 2?g/ml Rabbit Anti-M)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse APOB Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Product Categories/Family for anti-APOB antibody

Metabolic Pathway; Endocrinology; Cardiovascular Biology; Hepatology; Nutrition Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 161702988
• NCBI GeneID: 238055
• NCBI Accession #: NP_033823.2
• NCBI GenBank Nucleotide #: NM_009693.2
• UniProt Accession #: E9Q414; Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8
• Molecular Weight: 509,432 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: apob-48; AI315052; apob-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.