Human Thrombospondin Type I Domain Containing Protein 7A (THSD7A) ELISA Kit | THSD7A elisa kit

Human Thrombospondin Type I Domain Containing Protein 7A (THSD7A) ELISA Kit

Cat. Num. AAA456358

• Reactivity: Human
• Synonyms: Thrombospondin Type I Domain Containing Protein 7A (THSD7A); Human Thrombospondin Type I Domain Containing Protein 7A (THSD7A) ELISA Kit; THSD7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of THSD7A. No significant cross-reactivity or interference between THSD7A and analogues was observed.
• Sequence Length: 1,657

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level THSD7A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level THSD7A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for THSD7A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of THSD7A in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to THSD7A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to THSD7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain THSD7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of THSD7A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 150170672
• NCBI GeneID: 221981
• NCBI Accession #: NP_056019.1
• NCBI GenBank Nucleotide #: NM_015204.2
• UniProt Accession #: Q9UPZ6
• Molecular Weight: 185,363 Da
• NCBI Official Full Name: thrombospondin type-1 domain-containing protein 7A
• NCBI Official Synonym Full Names: thrombospondin type 1 domain containing 7A
• NCBI Official Symbol: THSD7A
• NCBI Protein Information: thrombospondin type-1 domain-containing protein 7A
• UniProt Protein Name: Thrombospondin type-1 domain-containing protein 7A
• Protein Family: Thrombospondin type-1 domain-containing protein
• UniProt Gene Name: THSD7A
• UniProt Synonym Gene Names: KIAA0960

NCBI Description

The protein encoded by this gene is found almost exclusively in endothelial cells from placenta and umbilical cord. The encoded protein appears to interact with alpha(V)beta(3) integrin and paxillin to inhibit endothelial cell migration and tube formation. This protein may be involved in cytoskeletal organization. Variations in this gene may be associated with low bone mineral density in osteoporosis. [provided by RefSeq, Aug 2010]

Uniprot Description

The soluble form promotes endothelial cell migration and filopodia formationduring angiogenesis via a FAK-dependent mechanism.