Mouse Apolipoprotein B (APOB) ELISA Kit | APOB elisa kit

Mouse Apolipoprotein B (APOB) Wide-range ELISA Kit

Cat. Num. AAA2088266

• Gene Names: Apob; apob-48; AI315052; apob-100; Apo B-100
• Reactivity: Mouse
• Synonyms: Apolipoprotein B (APOB); Mouse Apolipoprotein B (APOB) Wide-range ELISA Kit; Apo-B; FLDB; Apo B-100; Apo B-48; APOB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Apolipoprotein B (APOB).; No significant cross-reactivity or interference between Wide-range Apolipoprotein B (APOB) and analogues was observed.
• Sequence Length: 4505

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 31.2-2,000ng/mL
• Sensitivity: < 13.5ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Apolipoprotein B (APOB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Apolipoprotein B (APOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Apolipoprotein B (APOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for APOB elisa kit

Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 161702988
• NCBI GeneID: 238055
• NCBI Accession #: NP_033823.2
• NCBI GenBank Nucleotide #: NM_009693.2
• UniProt Accession #: E9Q414; Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8
• Molecular Weight: 509,432 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: apob-48; AI315052; apob-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12 A1.1|12 3.53 cM

Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; endoplasmic reticulum; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; vesicle membrane

Molecular Function: cholesterol transporter activity; heparin binding; lipid binding; low-density lipoprotein receptor binding; phospholipid binding

Biological Process: artery morphogenesis; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fertilization; in utero embryonic development; lipid catabolic process; lipid metabolic process; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; post-embryonic development; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; sperm motility; spermatogenesis; triacylglycerol catabolic process; triacylglycerol mobilization