Rabbit Apolipoprotein B (APOB) Polyclonal Antibody | anti-APOB antibody

Polyclonal Antibody to Apolipoprotein B (APOB)

Cat. Num. AAA2006107

• Gene Names: Apob; apob-48; AI315052; apob-100; Apo B-100
• Reactivity: Mus musculus (Mouse)
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Apolipoprotein B (APOB); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein B (APOB); anti-APOB antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-APOB antibody: Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Rat
• Immunogen: Recombinant APOB (Leu32~Met306) expressed in E.coli (MBS2105389).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot;Sample: Recombinant APOB, Mouse.)

NCBI and Uniprot Product Information

• NCBI GI #: 161702988
• NCBI GeneID: 238055
• NCBI Accession #: NP_033823.2
• NCBI GenBank Nucleotide #: NM_009693.2
• UniProt Accession #: E9Q414; Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: Apob
• NCBI Official Synonym Symbols: apob-48; AI315052; apob-100; Apo B-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apob
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12 A1.1|12 3.53 cM

Cellular Component: cell soma; chylomicron; cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum exit site; extracellular exosome; extracellular region; extracellular space; intermediate-density lipoprotein particle; intracellular membrane-bound organelle; low-density lipoprotein particle; mature chylomicron; very-low-density lipoprotein particle; vesicle membrane

Molecular Function: cholesterol transporter activity; heparin binding; lipid binding; lipid transporter activity; low-density lipoprotein receptor binding; phospholipid binding

Biological Process: artery morphogenesis; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fertilization; in utero embryonic development; lipid catabolic process; lipid metabolic process; lipid transport; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; low-density lipoprotein particle clearance; low-density lipoprotein particle remodeling; nervous system development; positive regulation of cholesterol storage; positive regulation of gene expression; positive regulation of lipid storage; positive regulation of macrophage derived foam cell differentiation; post-embryonic development; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; sperm motility; spermatogenesis; steroid metabolic process; transport; triacylglycerol mobilization; triglyceride catabolic process