Rat Apelin ELISA Kit | Apln elisa kit

Rat Apelin ELISA Kit

Cat. Num. AAA764107

• Gene Names: APLN; APEL; XNPEP2
• Reactivity: Rat
• Synonyms: Apelin; Rat Apelin ELISA Kit; Apln (Apelin)/APEL/XNPEP2/APJ endogenous ligand; Apln elisa kit

• Reactivity: Rat
• Sequence Length: 77

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Apln elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat Apln. During the reaction,Rat Apln in the sample or standard competes with a fixed amount of Rat Apln on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Rat Apln. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat Apln in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6708146
• NCBI GeneID: 8862
• Molecular Weight: 8,569 Da
• NCBI Official Full Name: apelin
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: APLN
• NCBI Official Synonym Symbols: APEL; XNPEP2
• NCBI Protein Information: apelin
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: APLN
• UniProt Synonym Gene Names: APEL
• UniProt Entry Name: APEL_HUMAN

NCBI Description

This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016]

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: apelin receptor binding; G-protein-coupled receptor binding; hormone activity; receptor binding

Biological Process: drinking behavior; immune response; lactation; negative regulation of blood pressure; negative regulation of vasoconstriction; positive regulation of adrenocorticotropic hormone secretion; positive regulation of cell proliferation; positive regulation of corticotropin-releasing hormone secretion; positive regulation of heat generation; positive regulation of phosphorylation; positive regulation of vasodilation; regulation of respiratory gaseous exchange; regulation of the force of heart contraction; signal transduction