Bovine Total Apelin ELISA Kit | Apelin elisa kit

Bovine Total Apelin ELISA Kit

Cat. Num. AAA738042

• Reactivity: Bovine
• Synonyms: Total Apelin; Bovine Total Apelin ELISA Kit; Apelin elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This APLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine APLN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apelin elisa kit

Principle of the Assay: APLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APLN antibody and an APLN-HRP conjugate. The assay sample and buffer are incubated together with APLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APLN concentration since APLN from samples and APLN-HRP conjugate compete for the anti-APLN antibody binding site. Since the number of sites is limited, as more sites are occupied by APLN from the sample, fewer sites are left to bind APLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APLN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Apelin elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 6708146
• Molecular Weight: 8,569 Da
• NCBI Official Full Name: apelin
• UniProt Protein Name: Apelin
• UniProt Gene Name: APLN
• UniProt Synonym Gene Names: APEL
• UniProt Entry Name: APEL_HUMAN

NCBI Description

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular space; extracellular region

Molecular Function: G-protein-coupled receptor binding; hormone activity; apelin receptor binding; receptor binding

Biological Process: lactation; immune response; signal transduction