Rat Total Apelin ELISA Kit | APELIN elisa kit

Rat Total Apelin ELISA Kit

Cat. Num. AAA25788

• Gene Names: APLN; APEL; XNPEP2
• Reactivity: Rat
• Synonyms: Total Apelin; Rat Total Apelin ELISA Kit; APELIN elisa kit

• Reactivity: Rat

• Sensitivity: 1.0 ng/ml.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APELIN elisa kit

INTRODUCTION ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentrationof the analyte in the sample.

INTENDED USE This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rat APELIN in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rat APELIN concentration. The concentration of Rat APELIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45120090
• NCBI GeneID: 8862
• NCBI Accession #: NP_059109.3
• NCBI GenBank Nucleotide #: NM_017413.4
• UniProt Accession #: Q9ULZ1; Q4VY08; Q8WU89
• Molecular Weight: 8,569 Da
• NCBI Official Full Name: apelin preproprotein
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: APLN
• NCBI Official Synonym Symbols: APEL; XNPEP2
• NCBI Protein Information: apelin; AGTRL1 ligand; APJ endogenous ligand
• UniProt Protein Name: Apelin
• UniProt Gene Name: APLN
• UniProt Synonym Gene Names: APEL
• UniProt Entry Name: APEL_HUMAN

NCBI Description

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular space; extracellular region

Molecular Function: G-protein-coupled receptor binding; hormone activity; apelin receptor binding; receptor binding

Biological Process: lactation; immune response; signal transduction